In life, it’s easy to turn on a light or adjust the light. But systems that achieve similar control of biomolecular functions are complex and poorly understood.
In biology, protein functions are turned on and off in complex ways. Allosteric regulation is one of the important biological regulatory mechanisms and is crucial for healthy metabolism and cell signaling. But creating allostery in synthetic protein systems has always presented significant challenges.
Recently, David Baker’s team at the University of Washington designed a protein that can switch between assembly and disassembly reliably and accurately through allosteric control. Using AI to design new proteins that do not exist in nature, researchers have engineered multiple dynamic protein arrangements.
David Baker said: "By designing proteins that can be assembled and disassembled on command, we are paving the way for future biotechnologies that may rival the complexity of nature."
# 🎜🎜#Arvind Pillai, first author and corresponding author of the paper, said: "One of the key innovations of this study is the design of protein assemblies that can switch between different oligomer states, such as dimers. , rings and cages in response to effector molecules, this ability to remotely control protein structure opens up the possibility of developing adaptive biomaterials and drug delivery systems. A stunning breakthrough in design." The relevant research was titled "De novo design of allosterically switchable protein assemblies" and was published in "Nature" on August 14.Allostery and de novo design
Paper link: https://www.nature.com/articles/s41586-024-07813-2#🎜 🎜#
De novo design
De novo designed proteins expand the repertoire of naturally evolved properties, opening the door to more controllable control of protein function.Design strategy
Research results:
Key Innovation:
A key innovation in this research is the design of protein assemblies. In addition to structural versatility, the team also achieved high-affinity binding between the new protein and its effectors, ensuring reliable programmed allosteric control. "For this project, we used specific peptides as effectors, but any type of molecule can be used in Protein allostery occurs under the right conditions," added co-author Abbas Idris, a graduate student at the University of Washington.Illustration: Design of allosterically controlled ring assembly. (Source: paper) The researchers synthesized a number of designed proteins and then characterized the protein structure and the switching behavior caused by the binding of effector molecules. Nearly 40% of synthetic proteins designed to switch between ring assemblies composed of varying numbers of protomers are water-soluble and display the expected protomer stoichiometry.
Furthermore, the number of effectors bound to a protein follows the MWC model: all binding sites are filled, or none at all. In other words, homologous effector binding is highly cooperative and the resulting assembly does not contain a mixture of R and T protomers.
Illustration: A protein switches between assembly states by design. (Source: Nature)
Going one step further, the researchers designed proteins containing double hinges (two hinges connected by short loops) with the goal of creating structures that respond to effector binding without changing the number of protomers in the protein assembly. And the protein changes its 3D structure. Sure enough, these proteins functioned as expected, reproducing the dominant behavior of naturally occurring allosteric proteins such as hemoglobin. Finally, the researchers also designed protomers that assemble or disassemble when bound to effector molecules.
Specific de novo protein assemblies designed in the study include rings formed by the dimerization of two monomers, which upon assembly trigger light output for biosensing applications, and cage-like structures, which undergo controlled disassembly for Release the payload for drug delivery. These protein dynamics were experimentally verified in vitro by size exclusion chromatography, mass spectrometry, and electron microscopy.
Pillai emphasized that ring structures exhibit additional precise properties such as cooperativity, a phenomenon exhibited by natural systems (e.g. blood proteins, hemoglobin). In synergistic systems, the binding of one molecule enhances the binding of other molecules, creating rapid on-off reactions that are critical for precise control, such as capturing oxygen in the lungs and releasing it into tissues.
"Historically, in the laboratory, we've done a lot to control the affinity of binding a substance, such as binding it tighter and tighter. But that's not the only aspect relevant to biological systems," Pillai said. "Sometimes you want to be able to bind over a very narrow concentration range." To validate the design, the researchers characterized more than 20 protein assemblies using negative staining and cryo-electron microscopy. "This allowed us to confirm which designs formed as expected and observe how these assemblies changed their structure when effector molecules were introduced," explains Dr. Andrew Borst, head of the IPD Electron Microscopy Research Core.
Illustration: Structure characterization of sr312 and sr322 by electron microscopy. (Source: paper) 
Designed components include nanoscale containers that can be opened and closed remotely. Such systems could lead to novel drug delivery vehicles with advanced control mechanisms, including devices that sequester cell-killing drugs until they encounter tumors.
This research paves the way for the design of allosterically controlled functions beyond protein assembly and disassembly, such as regulating enzyme activity for metabolic functions and nanomachines that can convert energy into mechanical work, similar to the proteins responsible for cell movement. Actin and myosin.
“The next step is to determine whether we can form interactions with small molecules and accurately catalyze reactions, which is a more challenging frontier for the entire field,” Pillai said.
Going forward, the research team seeks to evaluate these engineered protein dynamics in a broader biological context. Future work includes installing these engineered features on cell surfaces in tissue culture, providing valuable tools for feedback control in therapeutics such as adoptive cell therapy.
Reference content:
https://www.bakerlab.org/2024/08/14/morphing-protein-assemblies-by-design/The above is the detailed content of AI designs protein 'switches' from scratch, an amazing breakthrough in protein design, David Baker's research is published in Nature. For more information, please follow other related articles on the PHP Chinese website!
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